Coding

Part:BBa_K4131006:Design

Designed by: Chun Ying Yu   Group: iGEM22_CCU_Taiwan   (2022-10-09)


Original pfa D gene (Moritella marina)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 377
    Illegal PstI site found at 1079
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1302
    Illegal PstI site found at 377
    Illegal PstI site found at 1079
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 240
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 377
    Illegal PstI site found at 1079
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 377
    Illegal PstI site found at 1079
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No modification is made in the original sequence.


Source

The sequence is extracted from the region 1621982-1623598 in Moritella marina genome (WP_019440098.1)

References